Screening of Ornamental Flower Extracts as Natural Staining Agents

Antoniette G.  Bacaling; Merrose B.  Descarial; Nicanor S.  Togle; Juvy L.  Amable; Diloram Babajanovna  Babajanova

doi:10.51699/cajotas.v7i3.1690

Authors

Antoniette G. Bacaling Graduate School students PhD in Education major in Science Iloilo State University of Fisheries Science and Technology, Philippines
Merrose B. Descarial Graduate School students PhD in Education major in Science Iloilo State University of Fisheries Science and Technology, Philippines
Nicanor S. Togle Graduate School students PhD in Education major in Science Iloilo State University of Fisheries Science and Technology, Philippines
Juvy L. Amable Graduate School students PhD in Education major in Science Iloilo State University of Fisheries Science and Technology, Philippines
Diloram Babajanovna Babajanova Professor, Doctor of Historical Sciences, Uzbekistan State University of World Languages

DOI:

https://doi.org/10.51699/cajotas.v7i3.1690

Keywords:

Extracts, Natural Staining Agents, Ornamental Flower, Screening

Abstract

This study aimed to provide additional information using natural dyes as a substitute for synthetic dyes in the viewing of plant and animal cells. It utilized the quantitative descriptive experimental research design using standard laboratory procedures. An experimental method was used in the study: it started with collecting flowers, then extract preparation using mechanical extraction, plant cell preparation, animal cell preparation, and staining the specimen for microscopic observation. Basically, there were five (5) treatments, each treatment had 3 replications. The result of the study showed that Begonia x hybrida and Mirabilis jalapa can stain the nucleus, cell wall, and cytoplasm of the plant cell when viewed under 100x and 400x magnification. While in Thunbergia erecta, the parts of the plant cell cannot be distinctly seen. In contrast, none of the extracts successfully stained animal cells, suggesting their limited application in animal histology. Compared to synthetic dyes, which pose health and environmental risks, natural dyes offer an eco-friendly and biodegradable alternative. However, their selective staining ability emphasizes their potential use in plant histology rather than animal tissue staining. Further research is needed to enhance their staining efficiency and expand their applications in microscopy.

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References

H. Wagiyanti and R. Noor, “Red dragon fruit (Hylocereus costaricensis Britt. Et R.) Peel extract as a natural dye alternative in microscopic observation of plant tissues: The practical guide in senior high school,” Jurnal Pendidikan Biologi Indonesia, vol. 3, no. 3, pp. 232–237, 2017.

C. L. Green, Nutritional Colourant and Dyestuff: A review of Production Markets and Development Potential. Rome, Italy: Food and Agricultural Organization of the United Nations, 1995.

N. A. Olise, I. B. Enweani, I. O. Oshim, E. U. Urama, and C. C. Ngwoke, “The use of extracts from Nigerian indigenous plants as staining techniques in bacteriology, mycology, and histopathology,” Int. J. Plant Res, vol. 8, no. 1, pp. 15–24, 2018.

M. P. Rubina, A. M. Krishnan, K. B. Riyas Basheer, T. K. Mohammed Safeer, and V. Soumya, “Assessment of staining quality of curcumin as a substitute for eosin in hematoxylin and eosin staining in histopathology,” J Res Med Dent Sci, vol. 8, no. 5, pp. 146–150, 2020.

K. Deepali, S. Lalita, and M. Deepika, “Application of Aqueous Plant Extracts as Biological Stains,” Int. J. Sci. Eng. Res., vol. 5, no. 2, pp. 1587–1589, 2014.

C. C. Bondoc, “Curcuma longa Linn rhizome extract as an alternative stain for histological studies,” J. Pharmacogn. Phytochem., vol. 7, no. 5, pp. 3010–3017, 2018.

S. M. Adeyemo, A. J. Akinloye, and G. B. Adekanmi, “The Use of Plant Dyes for Microbial Staining and Identification: An Eco-friendly and Non-Toxic Alternative Method,” J. Adv. Biol. Biotechnol., vol. 16, no. 4, pp. 1–10, 2017.

A. J. Madanakumar and M. Kumaraswamy, “Purified anthocyanin, its elicitation from cell cultures of Begonia malabarica and Begonia rex-cultorum ‘Baby Rainbow’and it’s in vitro cytotoxicity Analysis by MTT Assay,” Pharmacognosy Journal, vol. 10, no. 3, 2018.

R. Rozina, “Pharmacological and biological activities of Mirabilis jalapa L.,” Int J Pharmacol Res, vol. 6, no. 5, pp. 160–168, 2016.

R. Bhuyan and C. N. Saikia, “Isolation of colour components from native dye-bearing plants in Northeastern India,” 2004.

H. J. R. Calilung, A. L. Castillo, C. A. U. Gasmido, C. Anne, S. Legaspi, and R. M. E. Castor, “Efficacy of Bougainvillea spectabilis (Bougainvillea) bracts crude extract as an alternative to Wright stain in blood smear preparation,” European Journal of Molecular & Clinical Medicine, vol. 7, no. 2, 2020.

C. R. Rathi and S. N. Suresh, “Mirabilis jalapa Flower Extract as Therapeutic Agent and Cellular Delivery by Nanoparticles,” Journal of Drug Delivery & Therapeutics, vol. 11, 2021.

R. Siva, “Status of natural dyes and dye-yielding plants in India,” Curr. Sci., pp. 916–925, 2007.

O. Uchejeso and O. Ejinaka, “Beetroot and turmeric as alternative dyes for haematoxylin and eosin in histological staining,” Sci Res J Clin Med Sci, vol. 2, pp. 54–88, 2022.

Ş. S. Bayazit, “Investigation of Safranin O adsorption on superparamagnetic iron oxide nanoparticles (SPION) and multi-wall carbon nanotube/SPION composites,” Desalination Water Treat., vol. 52, no. 37–39, pp. 6966–6975, 2014.